| ORIGINAL RESEARCH |
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| Year : 2023 | Volume
: 15
| Issue : 1 | Page : 89-96 |
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Comparative evaluation of the antibacterial efficacy of herbal agents as intracanal medicaments individually or in combination with chitosan: An in vitro RTPCR study
Gaurav Patri, Kotni Sheetal, Prasanti Kumar Pradhan, Pratik Agrawal, S Lata
Department of Conservative Dentistry and Endodontics, Kalinga Institute of Dental Sciences, KIIT Deemed to Be University, Bhubaneswar, Odisha, India
Correspondence Address:
Dr. Gaurav Patri
Department of Conservative Dentistry and Endodontics, Kalinga Institute of Dental Sciences, KIIT Deemed to Be University, Bhubaneswar, Odisha
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jioh.jioh_154_22
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Aim: To evaluate and compare the antimicrobial efficacy of herbal agents as intracanal medicaments with or without a carrier against E. faecalis. Materials and Methods: In this in vitro study, 80 extracted human single-rooted anterior or premolar teeth were used. They were decoronated to obtain standardized 6 mm blocks of the mid root. The internal root diameter was standardized(GG drill #3). All samples underwent 21-day contamination with E. faecalis after which they were randomly divided into(n = 20) Group 1, Triple antibiotic paste (Control); Group 2, Curcumin; Group 3, Propolis and Group 4, Aloe Vera gel. The groups were further sub grouped(n = 10) based on presence / absence of carrier (chitosan). 14 days post medication, E. faecalis from samples was quantified using qPCR and the data was analysed using One-way ANOVA and Tukeys Post-Hoc test. Results: All medicaments exhibited antimicrobial properties. TAP performed the best, Individually, Propolis showed almost similar efficacy (P = 0.598). With chitosan combination, Curcumin was a close contender to TAP (P = 0.963). Aloe Vera gave the poorest result, but on addition of chitosan there was a significant increase (P = 0.000) in the antimicrobial efficacy. Conclusion: Based on the aim and the results obtained it can be concluded that all the test medicaments exhibited antimicrobial properties. TAP had superior antimicrobial properties compared to others. Individually, propolis and in combination with chitosan, curcumin showed similar antimicrobial efficacy. Aloe vera performed the poorest but when combined with chitosan, its antimicrobial properties significantly improved. |
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